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New technique records neuronal teamwork in live mice

by  /  23 October 2019
Group of images of neurons glow green
Neuron lights: In the top row, neurons are labeled with a fluorescent probe that reveals electrical activity. In the bottom row, neurons are labeled with a variant of the probe that accumulates specifically in the neuron cell bodies, preventing interference from axons of neighboring neurons.

Courtesy of Ed Boyden / MIT

A new imaging technique detects specific groups of firing neurons in the brains of moving mice, enabling scientists to study brain activity linked to a particular behavior.

The researchers used a voltage-sensing molecule, called SomArchon, that causes active neurons to glow under a light microscope. The molecule can reveal the electrical activity of an average of 14 neurons at once — more neurons than usually possible with other methods.

The researchers were able to record neurons in the cortex, as well as regions deep in the brain such as the hippocampus and striatum, while the mice ran on a spherical treadmill. They presented the unpublished findings yesterday at the 2019 Society for Neuroscience annual meeting in Chicago, Illinois.

“SomArchon is usable with a very typical, conventional one-photon microscope, whereas other sensors require complex setups,” says Sanaya Shroff, a graduate student in Xue Han’s lab at Boston University who presented the work.

SomArchon is a modified version of another molecule, Archon, developed last year by researchers at the Massachusetts Institute of Technology. Both molecules embed in the membranes of neurons and glow fluorescent as the membrane voltage changes. Unlike its predecessor, SomArchon resides only in the membrane of the neurons’ cell bodies, minimizing the glare from neuronal branches.

Standard methods for recording neuronal activity involve inserting electrodes into the brain, but electrodes typically record from only a single neuron. Multielectrode arrays record from hundreds but pick up noise from neighboring neurons in densely packed areas.

With SomArchon, researchers can not only record from a population of neurons, but also genetically target specific subclasses of neurons. The work was published 9 October in Nature1.

Just like conventional electrodes, the technique can track fast-firing neurons. It also reveals voltage fluctuations in the membrane even before the neuron makes a spike, giving scientists a full view of a neuron’s behavior.

For more reports from the 2019 Society for Neuroscience annual meeting, please click here.


References:
  1. Piatkevich K.D. et al. Nature Epub ahead of print (2019) PubMed